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Multiplex-Ready Marker Technology™
The Molecular Plant Breeding CRC has developed a new technology that permits the low-cost, high throughput analysis of SSRs on an automated DNA fragment analyser. The technology provides a number of advantages over other methods including reduced marker deployment costs, substantially reduced PCR assay costs via multiplexing, and a high amenability to automation. The technology comprises a single-step, closed-tube multiplex PCR assay that combine the principles of the M13-tailed primer method (Oetting et al. 1995) and two-step multiplex PCR amplification (Belgrader et al. 1996).
Multiplex-Ready Marker Technology" facilitates faster, cheaper and higher-throughput automated multiplex PCR assays. This technology is being used or evaluated by several research groups and cereal breeding programs in Australia. To discuss commercial licensing opportunities, please contact Chris Coughlan, Business Development Manager, Molecular Plant Breeding CRC. Technical information such as performing the method, (i.e. the tag primer sequences) can be discussed with Dr Matt Hayden.
Further information about MRT is available from the information pack and published paper. Further information about Molecular Plant Breeding can be found at website: www.molecularplantbreeding.com.
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