||Comparative and Functional Genomics
||High-throughput SNP genotyping
||Jenkins S, Gibson N
|Volume and Page
Whole genome approaches using single nucleotide polymorphism (SNP) markers have
the potential to transform complex disease genetics and expedite pharmacogenetics research.
This has led to a requirement for high-throughput SNP genotyping platforms. Development of
a successful high-throughput genotyping platform depends on coupling reliable assay chemistry
with an appropriate detection system to maximise efficiency with respect to accuracy, speed
and cost. Current technology platforms are able to deliver throughputs in excess of 100 000
genotypes per day, with an accuracy of >99%,at a cost of 20-30 cents per genotype. In order
to meet the demands of the coming years, however,genotyping platforms need to deliver throughputs
in the order of one million genotypes per day at a cost of only a few cents per genotype.
In addition, DNA template requirements must be minimised such that hundreds of thousands of SNPs
can be interrogated using a relatively small amount of genomic DNA. As such, it is predicted that
the next generation of high-throughput genotyping platforms will exploit large-scale multiplex reactions
and solid phase assay detection systems.Copyright 2001 John Wiley and Sons, Ltd.
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