| Journal |
: |
Journal of Plant Biochemistry and Biotechnology |
| Title |
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Evaluation of SSR markers for the assessment of genetic diversity and fingerprinting of Gossypium hirsutum accessions |
| Authors |
: |
Arunita R, Rakshit S, Santhy V, Gotmare VP, Mohan P, Singh VV, Singh S, Singh J, Balyan HS, Gupta PK, Bhat SR |
| Volume and Page |
: |
19(2): n/a |
| Year |
: |
2010 |
 |
Abstract
A total 177 simple sequence repeat (SSR) markers were screened using a set of 47 Upland cotton genotypes comprising 14 commercial varieties, 14 germplasm accessions and 19 advanced breeding lines to identify informative markers for genetic diversity assessment and fingerprinting in G. hirsutum. Only 21% (38/177) of SSR markers tested showed polymorphism with a mean of 2.18 alleles per locus and with average polymorphism information content (PIC) of 0.32. The SSR markers revealed a Jaccard's similarity coefficient ranging between 0.43 and 0.89, with an average of 0.67 among accessions. Cluster analysis using unweighted pair group method with arithmetic averages (UPGMA) and principal component analysis (PCA) indicated that majority of the genotypes were very closely related. All the 47 genotypes showed heterozygosity for at least one of the SSR loci. We discovered 19 rare and 6 unique alleles among the tested genotypes of cotton. Fingerprint based on all the 38 loci revealed a probability of identical match by chance of 3.98×10−8. A set of ten SSR markers was identified which could distinguish all the 47 genotypes with a moderate probability of identical match by chance ( = 0.01).
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