The input to the primer redundancy package is a fasta file (.fasta extension) containing the set of sequences that needs to be checked for redundancy. This
analysis tool is built around the pairwise comparison algorithm with the restriction of no gap and no mismatch. MON SSR project data was not included in the primer redundancy analysis due to the lack of data on target repeat motifs.
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Processing Summary
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Number of primer sequences analyzed (forward and reverse)
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18,002
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Total number of redundant primer sequences found
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2,570
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Total number of non-redundant primer sequences
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15,432
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Threshold value for the analysis
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0.81 (81%)
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Total number of redundant primer pairs
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1,422
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Total number of completely matched sequence pairs (match percentage 100)
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940
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Total number of closely matched sequence pairs (match percentage 90-99)
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280
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Total number of closely matched sequence pairs (match percentage 81-89)
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202
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Type of primer sequence match (pairs):
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Forward-forward match
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460
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Reverse-reverse sequence match
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414
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Forward-reverse sequence match
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232
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Reverse-forward sequence match
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316
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Both forward-forward and reverse-reverse match
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103
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