BNL: Microsatellites Derived from Gossypium hirsutum cv. Delta Pine
90 Small Insert Enriched Genomic Libraries
Ben Burr, Michael Blewitt and Eileen Matz
Brookhaven National Laboratory
Upton, NY 11973 USA
- Project Description
In cotton, the first SSR markers were developed at Brookhaven
National Laboratory (prefix “BNL”). The BNL
microsatellites was derived from G. hirsutum cv. Delta
Pine 90 small insert genomic libraries
enriched for (GA/CT)n and (CA/GT)n inserts. DNA from 4,046 colonies
with positive hybridization signals was sequenced and primers were
made for 766 unique flanking sequences with inserts containing at
least 10 simple repeats. Primer pairs were tested against a panel
of Gossypium hirsitum and Gossypium barbadense cultivars and those
that that gave products for all cultivars and showed at least one
polymorphism were selected for further work. Of these, 74 primer
pairs failed, 303 were not polymorphic, and 389 were polymorphic.
of 379 primer pairs are presented in the CMD.
the standardized panel screened BNL marker data
- Download the BNL
primers (Excel format).
- Download the BNL
microsatellite sequences (fasta
- Search your sequences against the BNL microsatellites
using the BLAST or
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