BNL: Microsatellites Derived from Gossypium hirsutum cv. Delta Pine 90 Small Insert Enriched Genomic Libraries

• Contributors
  Ben Burr, Michael Blewitt and Eileen Matz
  Biology Department
  Brookhaven National Laboratory
  Upton, NY 11973 USA
  
  Phone 1-631-344-3396
  Fax 1-631-344-3407
  E-mail: burr@bnl.gov
  URL: http://ukcrop.net/perl/ace/search/CottonDB
  
  
• Project Description
  In cotton, the first SSR markers were developed at Brookhaven National Laboratory (prefix “BNL”). The BNL microsatellites was derived from G. hirsutum cv. Delta Pine 90 small insert genomic libraries enriched for (GA/CT)n and (CA/GT)n inserts. DNA from 4,046 colonies with positive hybridization signals was sequenced and primers were made for 766 unique flanking sequences with inserts containing at least 10 simple repeats. Primer pairs were tested against a panel of Gossypium hirsitum and Gossypium barbadense cultivars and those that that gave products for all cultivars and showed at least one polymorphism were selected for further work. Of these, 74 primer pairs failed, 303 were not polymorphic, and 389 were polymorphic. A total of 379 primer pairs are presented in the CMD.
  
  
    View / Download the standardized panel screened BNL marker data (375).
    Download the BNL primers (Excel format).
    Download the BNL microsatellite sequences (fasta format).
    Search your sequences against the BNL microsatellites using the BLAST or FASTA server.
    References
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