Projects

CM: Large-Scale Isolation and Characterization of Microsatellites from Complex Plant Genomes

• Contributors
  James P. Connell, Muhammad J. Iqbal, Tim Huizinga, Avutu S. Reddy

  Crop Biotechnology Center and Dept. of Soil and Crop Sciences, Texas A&M University, College Station, Texas 77843-2123, USA

  Suyata Pammi

  Crop Biotechnology Center and Dept. of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2123, USA

• Project Description
  This project is aimed at large-scale isolation and characterization of microsatellite sequences from complex plant genomes. Leaf genomic DNA from G. hirsutum L., var. Coker 312, was used. The novelty of the method applied is in the use of randomly sheared (nebulized) genomic DNA for adapter-ligation, rigorous removal of biotinylated oligos, and high-density colony blots for constructing enriched libraries. Over 500 cotton microsatellites were identified and sequenced using this procedure.
  
  
    Download the CM primers (Excel format).
    Download the CM microsatellite sequences (fasta format).
    Search your sequences against the CM primers using the BLAST or FASTA server.
    References
  • Connell JP, Pammi S, Iqbal MJ, Huizinga T, Reddy AS. 1998. A high throughput procedure for capturing microsatellites from complex plant genomes. Plant Molecular Biology Reporter. 16:341-349