Projects

MGHES: EST-SSR Marker Resources from the Gossypium hirsutum EST Database of GenBank

• Contributors
  
  Samina N. Qureshi, Department of Plant and Soil Science, P.O. Box 9555, Mississippi State University, Mississippi State, MS 39762

  Sukumar Saha, J.N. Jenkins, USDA-ARS, Crop Science Research Laboratory, P.O. Box 5367, Mississippi State, MS 39762.

  Ramesh V. Kantety, Department of Crop and Soil Sciences, 726 Bradfield Hall, Cornell University, Ithaca, NY 14853
  

• Project Description
  One hundred and thirty-three SSR-containing ESTs (EST-SSRs) were identified by analyzing 9,948 sequences belonging to Gossypium hirsutum L. in GenBank. Primers were designed for 84 of these EST-SSRs and were tested for their ability to amplify and detect diversity among three lines of G. hirsutum and one line of G. barbadense L. EST-SSR were called MGHES (M=developed at Mississippi, GH= G. hirsutum, E=EST, S=SSR). In this project information about the primer sequence, repeat motif, and the degree of polymorphism of cotton MGHES EST-SSR markers is reported. A cost-effective strategy to develop EST-SSR markers by exploiting EST databases is demonstrated for the first time in cotton.
  
  
    Download the MGHES marker data in excel fomat as provided by Dr Sukumar Saha.
    Download the MGHES primers (Excel format).
    Downlaod the MGHES microsatellite sequences (fasta format).
    Search your sequences against the MGHES primers using the BLAST or FASTA server.
    References
  • Abdurakhmonov IY, Buriev ZT, Saha S, Pepper AE, Musaev JA, Almatov A, Shermatov SE, Kushanov FN, Mavlonov GT, Reddy UK, Yu JZ, Jenkins JN, Kohel RJ, Abdukarimov A. 2007. Microsatellite markers associated with lint percentage trait in cotton, Gossypium hirsutum. Euphytica. 156(1-2): 141-156.
  • Qureshi SN, Saha S, Kantety RV, Jenkins JN. 2004. EST-SSR: A new class of genetic markers in cotton. Journal of Cotton Science. 8:112-123.