Projects

NAU: Development and Mapping of Microsatellite Markers in Tetraploid Cotton

• Contributors
  Tianzhen Zhang, Wangzhen Guo cotton@njau.edu.cn
  National Key Laboratory of Crop Genetics & Germplasm Enhancement/Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China
  
  
• Project Description
  Microsatellite simple-sequence repeats (SSR) for both EST-SSR and Genomic-SSR was and/or is being developed in cotton. EST-SSRs are based on publicly available ESTs and sequences developed at CRI/NAU, while Genomic-SSRs are being developed from our BAC-end sequences in our G. hirsutum and G. arboretum BACs. All SSR information provides primer sequences, polymorphisms between TM-1 (Upland cotton standard) and Hai 7124 (G. barbadense) and chromosome localization based on our BC1 mapping population. “NAU” was used as a short prefix for Nanjing Agricultural University.
  
  
    Download the NAU marker data (Excel format).
    Download the NAU primer sequences (Excel format).
    Download the NAU microsatellite sequences (fasta format).
    Search your sequences against the NAU microsatellites using the BLAST or FASTA server.
    References
  • Guo W, Cai C, Wang C, Han Z, Song X, Wang K, Niu X, Wang C, Lu K, Shi B, Zhang T. 2007.
    A microsatellite-based, gene-rich linkage map reveals genome structure, function, and evolution in Gossypium. Genetics. 176(1): 527-541.
  • Guo WZ, Sang ZQ, Zhou BL, Zhang TZ. 2007. Genetic relationships of D-genome species based on two types of EST-SSR markers derived from G. arboreum and G. raimondii in Gossypium. Plant Science. 172(4): 808-814.
  • Lacape JM, Dessauw D, Rajab M, Noyer JL, Hau B. 2007. Microsatellite diversity in tetraploid Gossypium germplasm: assembling a highly informative genotyping set of cotton SSRs. Molecular Breeding.
    19(1): 45-58.
  • Wang K, Guo W, Zhang T. 2007. Development of one set of chromosome-specific microsatellite-containing BACs and their physical mapping in Gossypium hirsutum L. Theoretical and Applied Genetics. 115(5): 675-682.
  • Guo W, Wang W, Zhou B, Zhang T. 2006. Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Theoretical and Applied Genetics. 112(8): 1573-1581.
  • Han Z, Wang C, Song X, Guo W, Gou J, Li C, Chen X, Zhang T. 2006. Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton. Theoretical and Applied Genetics. 112(3): 430-439.
  • Shen X, Guo W, Zhu X, Yuan Y, Yu JZ, Kohel RJ, Zhang T. 2005. Molecular mapping of QTLs for fiber qualities in three diverse lines in Upland cotton using SSR markers. Molecular Breeding. 15(2):169-181.
  • Song XL, Wang K, Guo W, Zhang J, Zhang T, Scoles GJ. 2005. A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L. and Gossypium barbadense L. Genome. 48(3): 378-390.
  • Han ZG, Guo WZ, Song XL, Zhang TZ. 2004. Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton. Molecular Genetics and Genomics. 272(3):308-327.
  • Zhang TZ, Yuan Y, Yu J, Guo W, Kohel RJ. 2003. Molecular tagging of a major QTL for fiber strength in Upland cotton and its marker-assisted selection. Theoretical and Applied Genetics. 106:262-268.
  • Liu L, Guo WZ, Zhu X, Zhang T. 2003. Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum L..Theoretical and Applied Genetics. 106: 461-469.
  • Zhang J, Guo W, Zhang T. 2002. Molecular linkage map of allotetraploid cotton (Gossypium hirsutum L. x Gossypium barbadense L.) with a haploid population. Theoretical and Applied Genetics. 105:1166-1174.